Conservation genetics utilizes the tools and concepts of genetics and applies them to problems in conservation biology. For example, molecular genetic techniques, such as protein electrophoresis, and analysis of mitochondrial DNA and highly variable nuclear genes (including DNA fingerprinting), have been important in documenting the extent and pattern of genetic variation in endangered species. We review these techniques and their advantages and disadvantages, and give examples of their application to endangered species. For captive animal populations, pedigree analysis has become the basic approach to evaluate breeding priority of particular individuals. Several pedigree analysis techniques are commonly used, but peeling and gene dropping give the most information. We compared these techniques and illustrate their value with applications to the Guam Rail, Przewalski's horse, and other endangered captive animals. The rationale for much conservation genetic interpretation is base in evolutionary genetics. We discuss the avoidance of inbreeding depression and the maintenance of genetic variation–both primary conservation genetic goals–from this perspective. In addition, we suggest aspects of these factors that deserve greater attention in their overall application to conservation planning. Finally, we briefly mention three evolutionary topics–the relationship of heterozygosity and fitness, population bottlenecks, and outbreeding depression–that have implications for conservation genetics. Although simple interpretation in these areas is appealing, we feel that because they are only generally understood and often quite controversial, their application to endangered—species management should be carefully evaluated and monitored.