This paper gives a detailed analysis of the applied biosecurity measures in the production of boar sperm at a swine reproduction center. Biosecurity indicators (existence of a written biosecurity plan, isolation, introduction of newly acquired animals into the herd, herd health, assessment of the personnel attitude towards equipment, traffic control, attitude towards visitors, feeding and watering control, manure management, disposal of dead animal carcasses, attitude towards other animals, rodents and birds control, sanitation) were viewed and evaluated by rating scale: (5) - excellent, (4) - very good, (3) - good, (2) - sufficient, (1) - insufficient, there are resources for improvement (0) - insufficient, with no resources for improvement. Obtained data were analyzed in the SWOT process, taking into account all the strengths, weaknesses, threats and opportunities for improving the biosecurity level. The situation in the center is rated as very good, with an average rating of 4.15. However, one disadvantage is serious and related to the boar facilities isolation possibilities, taking into account their location and the presence of two types of male breeding animals (boars and bulls) in the same location. Newly acquired breeding animals are purchased from various sources, but with a rigorous regime of control and not at the same time.