Comparison of α-amylase isoforms from the midgut of Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae developed in the wild and on an artificial diet

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2010 Serbia English Publisher:National Library of SerbiaJournal:Archives of Biological Sciences, volume 62, pages 575-584 (issn: 0354-4664, eissn: 1821-4339,

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Authors: Biljana Dojnov; N. Loncar; Natasa Bozic; Vera Nenadovic; Jelisaveta Ivanovic; Z. Vujcic;

doi: 10.2298/abs1003575d

Comparison of α-amylase isoforms from the midgut of Cerambyx cerdo L. (Coleoptera: Cerambycidae) larvae developed in the wild and on an artificial diet

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Abstract

?-Amylase isoforms of Cerambyx cerdo larvae from the wild (ML and SL) and reared in the laboratory (ADL) were compared. Three amylase isoforms were presented in the SL and ML extracts while two isoforms were presented in the ADL according to zymogram after isoelectric focusing (IEF). All C. cerdo amylase isoforms were acidic proteins (pI < 3.5). Seven amylase isoforms (ACC 1-7) from the midgut of C. cerdo larvae were found in the ML midgut extract, six in the SL extract, and four in the ADL extract according to native PAGE zymogram. The ADL amylase had the highest activity. All crude midgut extracts of C. cerdo larvae were fractionated on a Superose 12 HR column. The molecular mass of the ACC was estimated to be 34 kDa. .

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Serbia

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Keywords

α-amylase, xylophagous larvae, QH301-705.5, Cerambycidae, midgut, isoform, Cerambyx cerdo, Biology (General), zymogram

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