Sunflower (Helianthus annuus, L.) is an important oil seed crop, which ranks a fourth next to groundnut, soybean and rapeseed which contributes as edible oils over the world. Due to the importance of this crop, this study was conducted to investigate the effect of three levels of nitrogen fertilizer (30, 45 and 60 kg fad.-1) and eight genotypes of sunflower i.e. two cultivars (Giza 102 and Sakha-53) as control and six genotypes (A-120, A-34, A-44, A-45, A-47 and A-48) under North Sinai conditions through two seasons of 2015 and 2016. In this study there were 24 treatments including three nitrogen fertilizer levels and eight sunflower genotypes. Results showed that increasing nitrogen levels increased yield and it′s components and protein content, while, oil content was decreased. The interaction between Giza 102 and nitrogen level 60 kg N fad.-1gave high value in each of 100-seed weight, head seed weight, oil yield, seed yield and protein yield in 2015 season, but, the interaction between the fertilizer level 60 kg N fad.-1 and genotype A-48 gave significant superiority in 100 seed weight, head seed weight, oil yield, seed yield and protein yield in the 2016 season, In conclusion, sowing A-48 genotype with 60 kg N fad-1 was the best treatment under North Sinai conditions.