Low- fat cake prepared by partial replacement of shortening with Maltodextrin or Simplesse were organoleptically evaluated and subjected to analysis for gross composition and caloric value. A nutrition experiment on rats was conducted to determine the effect of low – fat cake diet on growth parameters and serum lipid profile. The sensory evaluation results indicated that no significant difference (P≤ 0.05) was found among replacement treatments up to 50% replacement level and the full fat cake, and the panelists considered that, all treatments were acceptable up to 75% replacement level. The results of gross chemical composition and caloric value showed a significant decreased (P≤ 0.05) in fat content and caloric value by increasing fat replacement level. The final body weight, weight gain, feed efficiency ratio and the organ weight (% of body weight) of rats were not significantly (P≤ 0.05) affected by fat replacement. Triglycerides (T.G), total cholesterol and low density lipoprotein cholesterol (LDL-C) of serum were significantly (P≤ 0.05) decreased by replacement 50% or 75% of shortening using both fat replacers. The calculated Atherogenic Index was significantly decreased (P≤ 0.05) by fat replacement at ratio of 25%, 50% and 75% by using maltodextrin or at ratio of 50% and 75% by simplesse. Low- fat cake prepared by partial replacement of shortening with Maltodextrin or Simplesse were organoleptically evaluated and subjected to analysis for gross composition and caloric value. A nutrition experiment on rats was conducted to determine the effect of low – fat cake diet on growth parameters and serum lipid profile. The sensory evaluation results indicated that no significant difference (P≤ 0.05) was found among replacement treatments up to 50% replacement level and the full fat cake, and the panelists considered that, all treatments were acceptable up to 75% replacement level. The results of gross chemical composition and caloric value showed a significant decreased (P≤ 0.05) in fat content and caloric value by increasing fat replacement level. The final body weight, weight gain, feed efficiency ratio and the organ weight (% of body weight) of rats were not significantly (P≤ 0.05) affected by fat replacement. Triglycerides (T.G), total cholesterol and low density lipoprotein cholesterol (LDL-C) of serum were significantly (P≤ 0.05) decreased by replacement 50% or 75% of shortening using both fat replacers. The calculated Atherogenic Index was significantly decreased (P≤ 0.05) by fat replacement at ratio of 25%, 50% and 75% by using maltodextrin or at ratio of 50% and 75% by simplesse.