Bovine ephemeral fever (BEF) virus belonging to Ephemerovirus genus of the Rhabdoviridae family causes aneconomically important seasonal acute vector- borne disease. Routine vaccination, is one of the main controlling steps,particularly in countries where the disease is endemic. This study deal with comparative evaluation of humeral immuneresponse of cattle using SNT and ELISA to three inactivated BEF vaccines. Inactivated BEF vaccine with Montanide ISA206 (water-in-oil-in-water) adjuvant given in 2 doses one month apart gave high protective antibody titer lasted for 44weeks’ post vaccination (WPV). The inactivated BEF vaccine with aluminum hydroxide gel adjuvant given in 2 dosesone month apart gave satisfactory immune response lasted for 30 WPV then neutralizing antibodies waned rapidly. Liveattenuated BEF vaccine that inactivated by saponin on time of vaccination give higher antibody titer which rise morerapidly lasted for 38 WPV only. It could be concluding that the inactivated BEF vaccine with Montanide ISA 206 adjuvantgave satisfactory higher and longer lasting antibody titer for 44 WPV and it was recommended to use this vaccine inendemic and non-endemic countries to control and prevent the disease especially before summer season