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BioTechniques
Article . 2011 . Peer-reviewed
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BioTechniques
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BioTechniques
Article . 2011
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BioTechniques
Article . 2011
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https://dx.doi.org/10.2144/000...
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High Transfection Efficiency of Neural Stem Cells with Magnetofection

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 2011 English Publisher:Informa UK LimitedJournal:BioTechniques, volume 50, pages 187-189 (issn: 0736-6205, eissn: 1940-9818, Copyright policy )
Authors: Cédric Sapet; Nicolas Laurent; Antoine de Chevigny; Loïc Le Gourrierec; Elodie Bertosio; Olivier Zelphati; Christophe Béclin;

doi: 10.2144/000113628

pmid: 21486240

High Transfection Efficiency of Neural Stem Cells with Magnetofection

Abstract

Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.

Related Organizations
Keywords

NeuroMag, QH301-705.5, Neurogenesis, DNA, Transfection, NSC, Magnetics, Mice, transfection, Neural Stem Cells, Magnetofection, Animals, Biology (General), neuronal differentiation, Cells, Cultured, neural stem cells

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    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 35
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 10%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
35
Top 10%
Top 10%
Top 10%
gold