Abstract Environmental monitoring networks such as the Integrated Carbon Observation System (ICOS) in Europe, the National Ecological Observatory Network (NEON) in the U.S., or the Terrestrial Ecosystem Research Network (TERN) in Australia deploy different sampling schemes for in situ measurements. We report on the intercomparison of measurements of the canopy gap fraction with different digital hemispherical photography setups adopting ICOS, NEON, and TERN sampling schemes. The test was carried out at the Järvselja Radiation Transfer Model Intercomparison (RAMI) birch stand. Results show that spreading out sampling points which cover more of the plot is important for a good representation of the forest as a whole. The NEON tower plot layout scheme may be more prone to errors in overall canopy properties estimation than ICOS or TERN due to its compact sampling layout and should always be used in conjunction with its distributed plots. Different camera setups involving different camera operators, camera bodies, lenses and settings yield slightly varied results, and it is important to ensure that the images are taken in such a way that they would not be over or underexposed, or out of focus. As a conclusion we recommend always to carry out intercomparison measurements with old and new cameras when devices are upgraded. Our study contributes towards establishing the uncertainty and evaluating potential error budget stemming from collecting in situ measurements using different sampling schemes and camera setups.