Dense volumes of duplex stainless steel are manufactured by directed energy deposition. Compact tension specimens are machined from these volumes in order to evaluate the fracture toughness in two directions : parallel or perpendicular to the deposited layers. Different values are measured in the two cases. In order to understand this anisotropy, additional analyzes are performed on the cracked specimens post-mortem. A classical metallography analysis reveals the highly oriented structure of the material, as well as phase localization. The study of the fracture surface reveals several points. At the macroscale, while the crack surfaces are flat in the parallel case, pronounced shear lips cover half of the fracture surface in the perpendicular case. At the microscale, fracture is ruled by microvoid coalescence. The mesoscale, which is inherited from the deposition strategy, is found to pilot the crack growth. The border between the primary solidified melt pools and the heat-affected zones, which corresponds to the interface between the deposited layers, is the preferred area for crack growth. Analyzing the crack surface roughness confirms the dominance of the mesoscale, as its characteristic lengthscale is retrieved. This explains the differences observed for the two tested directions of fracture: in the parallel case, the crack is aligned with the weak interfaces between layers, which channel the crack growth; in the orthogonal one, out-of-plane excursion of the crack becomes possible allowing the crack to follow a tortuous three-dimensional path that results in a higher toughness than in the parallel situation.