Bermudagrasses, Cynodon spp., periodically sustain winter injury in the transition zone between warm‐ and cool‐season turfgrasses. Our objective was to determine relative freeze tolerance levels of advanced lines, recently released cultivars, and standard varieties by means of laboratory‐based methodology. Freeze tolerance evaluations were divided into three groups on the basis of intended use. The vegetatively propagated fairway types (and their freeze tolerance values) included ‘GN‐1’ (−5.9°C), ‘Baby’ (−6.7°C), ‘Tifway’ (−6.7°C), ‘TifSport’ (−7.2°C), ‘Quickstand’ (−8.0°C), and ‘Midlawn’(−8.4°C). GN‐1 was significantly less hardy than TifSport, Quickstand and Midlawn. The second set of bermudagrasses comprised the seed‐propagated varieties: ‘Arizona Common’ (−5.6°C), ‘Mirage’ (−6.1°C), ‘Jackpot’ (−6.3°C), ‘Guymon’ (−7.4°C), and ‘Yukon’ (−7.6°C). Arizona Common was significantly less freeze tolerant than Guymon and Yukon. Mirage and Jackpot were not significantly hardier than Arizona Common. The third series of plants included vegetatively propagated bermudagrasses used for putting greens: ‘Champion’ (−4.8°C), ‘Floradwarf’ (−4.9°C), ‘MS‐Supreme’ (−5.2°C), ‘MiniVerde’(−5.8°C), ‘Tifeagle’ (−6.0°C), ‘Tifdwarf’ (−6.5°C), and ‘Tifgreen’ (−6.5°C). Tifdwarf and Tifgreen were significantly hardier than all of the other putting green bermudagrasses tested except for Tifeagle. Results should be useful in selecting appropriate genotypes for the transition zone of turfgrass adaptation.