Bermudagrass, Cynodon dactylon (L.) Pers., suffers periodic severe winter‐kill throughout much of its area of use in the contiguous USA. A research goal is to increase freeze tolerance in cultivars to lessen the risk of such damage. An identified research need is for Cynodon germplasm resources to be characterized for freeze tolerance and hybridization potential. Accordingly, the objective of this research was to characterize the relative freeze tolerance of selected fertile bermudagrass plants. Nine tetraploid (2n = 4x = 36) C. dactylon and two triploid (2n = 3x = 27) hybrid (C. dactylon × C. transvaalensis Burtt Davy) clonal plants (standards) were evaluated in two experiments. Plants were propagated clonally and established in Cone‐tainers (Ray Leach Cone‐tainer Nursery, Canby, OR) for about 10 wk. Acclimation took place for 4 wk in controlled environment chambers at 8/2°C (day/night) temperatures with a 10‐h photoperiod. Following acclimation, Cone‐tainers were placed into a freeze chamber and cooled rapidly to −2°C, induced to freeze with ice chips, then held overnight at −2°C. The freeze chamber was then programmed to cool linearly at 1°C per hour. For each cultivar, three Cone‐tainers were removed at each test temperature. Following thawing, Cone‐tainers were transferred to a greenhouse and regrowth was evaluated visually. Nonlinear regression was used to estimate Tmid, which corresponded to the midpoint of the sigmoidal response curve of survival vs temperature. Within experiment one, Tifgreen (Tmid = −7.2°C) was significantly less cold hardy than Quickstand (−9.0°C), A‐12204 (−9.2°C), Midiron (−9.9°C), and A‐12195 (−10.5°C). A‐12195 was significantly hardier than all genotypes except Midiron. In the second experiment, Arizona Common (−6.6°C), Tifgreen (−7.1°C), and A‐12205 (−7.1°C) were less hardy than A‐9959 (−8.7°C), A‐12156 (−8.9°C), A‐12198 (−9.5°C), and Midiron (−10.0°C). Midiron was hardier than all genotypes except A‐12198. The range of test temperatures chosen did not allow estimate of a Tmid value for Zebra, but nearly 50% of the plants were killed at −6.0°C.