The green industry has identified the use of biodegradable containers as an alternative to plastic containers as a way to improve the sustainability of current production systems. Field trials were conducted to evaluate the performance of four types of 1-gal nursery biocontainers [keratin (KR), wood pulp (WP), fabric (FB), and coir fiber (Coir)] in comparison with standard black plastic (Plastic) containers on substrate temperature, water use, and biomass production in aboveground nurseries. Locations in Kentucky, Michigan, Mississippi, and Texas were selected to conduct experiments during May to Oct. 2012 using ‘Green Velvet’ boxwood ( Buxus sempervirens × B. microphylla ) and ‘Dark Knight’ bluebeard ( Caryopteris × clandonensis ) in 2013. In this article, we were focusing on the impact of alternative container materials on hourly substrate temperature variations and plant growth. Substrate temperature was on an average higher (about 6 °C) in Plastic containers (about 36 °C) compared with that in WP, FB, and Coir containers. However, substrate temperature in KR containers was similar to Plastic. Substrate temperature was also influenced by local weather conditions with the highest substrate temperatures recorded in Texas followed by Kentucky, Mississippi, and Michigan. Laboratory and controlled environment trials using test containers were conducted in Kentucky to evaluate sidewall porosity and evaporation loss to confirm field observations. Substrate temperature was similar under laboratory simulation compared with field studies with the highest substrate temperature observed in Plastic and KR, intermediate in WP and lowest in FB and Coir. Side wall temperature was higher in Plastic, KR, and FB compared with WP and Coir, while side wall water loss was greatest in FB, intermediate in WP and Coir, and lowest in plastic and KR. These observations suggest that the contribution of sidewall water loss to overall container evapotranspiration has a major influence on reducing substrate temperature. The porous nature of some of the alternative containers increased water use, but reduced heat stress and enhanced plant survival under hot summer conditions. The greater drying rate of alterative containers especially in hot and dry locations could demand increased irrigation volume, more frequent irrigation, or both, which could adversely affect the economic and environmental sustainability of alternative containers.