Testing of real driving emissions (RDE) with portable emission measuring system (PEMS) in an appropriate road circuit became an obligatory element of new type approval of passenger cars since September 2017. In several projects the Laboratory for Exhaust Emissions Control (AFHB) of the Berne University of Applied Sciences (BFH) performed comparisons on passenger cars with different PEMS’s on chassis dynamometer and on road, considering the quality and the correlations of results. Particle number measuring systems (PN PEMS) were also included in the tests. The present paper informs about influences of E85 on RDE on two flex-fuel-vehicles, discusses some aspects of different ways of evaluation with different programs, shows comparison of different types of PN PEMS and represents the effects of simulation of slope on the chassis dynamometer.