Neuropsychologists pay much attention to career guidance for young people who are faced with the challenge of choosing the profession. Numerous studies on motivation and professional determination prove the need to identify individual features of the brain organization of mental functions in the context of psychological support for the educational process at school and career guidance. Neuropsychological research indicates the links between the predominant activity of a certain hemisphere of the brain and professional realization in certain areas. The empirical study was conducted using reliable and valid psycho-diagnostic techniques (“Questionnaire of professional self-realization” by O. M. Kokun (2014; 2016); the “Motivational profile” technique by S. Richie, & P. Martin (2004); correlation analysis). At the stage of qualitative analysis, two groups of subjects with different levels of professional self-realization were identified (using the «ace» method). A visual analysis of the motivational factors profile structure in groups with high and low levels of professional self-realisation demonstrated differences in the graphs configuration and their location, and also provided an opportunity to characterize the psychological motivation characteristics of representatives of each of the groups. Against the general background of communicative self-sufficiency, adaptability and self-confidence, in a group of persons with a high level of professional self-realisation, the dominant motives are constant improvement, recognition by others. Representatives of a group with a low level of the studied phenomenon are interested in the motives of good working conditions and high wages. It has been proved that persons with different levels of professional self-realization differ in the specificity of the dominance of motives.