The Nepomorpha and Gerromorpha heteropterans are highly diverse, mainly in Neotropical aquatic ecosystems; however, their composition in the lakes of the Tropical Andes is poorly known. This article presents the composition of aquatic and semiaquatic bugs and their geographical and altitudinal distribution in eight lentic ecosystems from the Cundinamarca department, Colombia. The similar aquatic heteropteran composition between these Andean lakes and its relationship with the geographical and altitudinal range from 1,585 to 3,700 m.a.s.l. in the Eastern Andes are also analyzed. To carry out this work, the collections and pertinent literature are reviewed, and biota similarity is compared by applying a upgma analysis. The results show the predominance of Nepomorpha with a total of 12 species, four genera, and two families: Corixidae (6), and Notonectidae (6). The second most common suborder is Gerromorpha with eight species, seven genera, and five families: Gerridae (3), Veliidae (2), Hebridae (1), Hydrometridae (1), and Mesoveliidae (1). The biota similarity is related to páramo ecosystems and forest type. This article is the first to consider aquatic and semiaquatic heteropterans as biota from the Andean and High Andean lakes in Colombia.