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Revista de Investigaciones Altoandinas
Article . 2018 . Peer-reviewed
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Revista de Investigaciones Altoandinas
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Efecto del ácido naftalenacético y 6-bencilaminopurina en la inducción de callos embriogénicos en anteras de Carica papaya L. variedad criolla

EFFECT OF NAFTALENACETIC ACID AND 6-BENCILAMINOPURINE IN THE INDUCTION OF EMBRIOGENIC CALLLUS OF Carica papaya L. var. criolla
Authors: Chico-Ruiz, Julio; Gonzales-Zavaleta, Osmar;

Efecto del ácido naftalenacético y 6-bencilaminopurina en la inducción de callos embriogénicos en anteras de Carica papaya L. variedad criolla

Abstract

El papayo ( Carica papaya L.), Caricaceae, es un cultivo importante por su alto valor nutricional y sus aplicaciones medicinales e industriales, sin embargo, este cultivo tiene problemas de plagas y enfermedades por lo cual hacen falta investigaciones para obtener variedades mejoradas de papaya. Una alternativa es el cultivo de anteras para lo cual es necesario conocer su competencia con la induccion de callos; con lo planteado el objetivo de esta experiencia fue determinar el efecto del acido naftalenacetico y 6-bencilaminopurina en la induccion de callos embriogenicos en anteras de C. papaya L. variedad criolla. Los pimpollos obtenidos fueron medidos y agrupados segun su longitud, para determinar el estado de desarrollo del grano de polen y se confirmo el estado de microspora con la coloracion de carmin acetico y HCl. Luego los explantes fueron sometidos a un pretratamiento en frio (6°C) por 4 dias, luego se sembraron en el medio Murashige & Skoog, que contenia sacarosa (3%), fitagel (0.5%), pH de 6.0 y combinaciones de ANA y BAP a concentraciones de ppm. La evaluacion se realizo durante 4 meses, el tratamiento T4 (ANA 1 y BAP 0.5) fue el mejor para el porcentaje de anteras con callos (hasta un 40%), en cuanto a microsporas inducidas el mejor fue T5 (ANA 1 y BAP 1 ) con el 2.9%. El tamano de las anteras aumento y el color de las mismas cambio de amarillo a marron. Durante el proceso de induccion no se observaron embriones, pero si desdiferenciacion del tejido (meristemoides). naftalenacetico (ANA) y 6-bencilaminopurina (BAP) ABSTRACT The papaya ( Carica papaya L.), Caricaceae, is an important crop for its high nutritional value and its medicinal and industrial applications, however, this crop has problems of pests and diseases, which requires research to obtain improved varieties of papaya. An alternative is the cultivation of anthers for which it is necessary to know their competence with the induction of calluses; with the stated objective of this experience was to determine the effect of naphthaleneacetic acid and 6-benzylaminopurine in the induction of embryogenic calluses in anthers of C. papaya L. var. criolla. The obtained buds were measured and grouped according to their length, to determine the state of development of the pollen grain and the microspore state was confirmed with the coloring of acetic carmine and HCl. Then the explants were subjected to a cold pretreatment (6 ° C) for 4 days, then they were seeded in the Murashige & Skoog medium, which contained sucrose (3%), phytagel (0.5%), pH 6.0 and combinations of ANA and BAP at ppm concentrations. The evaluation was performed during 4 months, the treatment T4 (ANA 1 and BAP 0.5) was the best for the percentage of anthers with calluses (up to 40%), as for microspores induced the best was T5 (ANA 1 and BAP 1 ) with 2.9%. The size of the anthers increased and the color of the anthers changed from yellow to brown. During the induction process, embryos were not observed, but tissue dedifferentiation (meristemoids) was observed. Key words: Carica papaya , anthers, microspores, naphthaleneacetic acid (ANA) and 6-benzylaminopurine (BAP)

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Keywords

naphthaleneacetic acid (ANA), ácido naftalenacético (ANA), Carica papaya, anteras, 6- bencilaminopurina (BAP), anthers, microspores, 6-benzylaminopurine (BAP), microsporas

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
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