<p>Fish oil could be extrated from lemuru (Sardinella lemuru) or lemuru canning industry by<br />products. The fish oil should be refined first before using as omega-3 sources. This reasearch<br />obtained was purification of fish oil from lemuru canning industry by products at Bali using<br />variations purification method. The fisrt step was done by analysis of crude oil such of free fatty<br />acids value, peroxide value, and iodine value. Then after the purification process using variations<br />purification method, the refined fish oil was analyzed for same parametrs. The best refined fish<br />oil was analyzed of composition fatty acid using gas chromatograph (GC) instrument. The result<br />showed that the crude oil had free fatty acids value, peroxide value, and iodine value as follows<br />24.03%; 6.97 meq/kg sample; 189.13 g/100 g sample. After the refining process using four methods,<br />the result showed that free fatty acids value, peroxide value, and iodine value became: the first<br />method 24.02%; 6.16 meq/kg sample; 187.91 g/100 g sample. The second method 23.14%; 4.17<br />meq/kg sample; 193.94 g/100 g sample. The third method 9.38%; 4.88 meq/kg sample; 225.39 </p><p>g/100 g sample. And the fourth method 11.03%; 5.64 meq/kg sample; 222.69 g/100 g sample. Due<br />to the peroxide value, the refined lemuru oil that could met standard of Indonesian farmacope for<br />consumed fish oil was resulted from the third method. In the refined lemuru oil could be found of<br />EPA component (Methyl cis-5,8,11,14,17-Eicosapentaenoic acid methyl ester) with concentration<br />650,65 μg/mL..<br />Keywords: By products, fish oil, refining process, Sardinella lemuru</p>