The effect of growing conditions on starch and protein content in triticale grain and amylose content in starch was studied on winter triticale cvs. Kitaro, Lupus, Lamberto and Ticino harvested in 2006 and 2007 in Humpolec and Pernolec, Czech Republic. Dry matter of the investigated triticale grain contained 62.4-70.9% of starch. The starch content and amylose content in starch were significantly affected by cultivar and year. Statistical analysis did not confirm that starch content and amylose content in starch were significantly influenced by growing variant or location. The protein content was significantly affected only by year. The differences in protein content among cultivars, growing variants and locations were not significant. The protein content was negatively correlated with starch content (r = -0.83). Statistical analysis of data also confirmed a positive correlation between the starch content and amylose content in starch (r = 0.45).