Grapevine (Vitis vinifera L.) is one of the most important fruit crops in Turkey and viral diseases are one of the main problems of grapevine production. They decrease yield quantity and quality of crops as well as cause death of the plants in an interaction with other agents. Leafroll disease of grapevine, caused by a complex of viruses, is considered a serious threat to production across many grapevine growing regions in Turkey. This study was conducted to determine the current status of Grapevine leafroll-associated viruses (GLRaVs) in Malatya province. Grapevine plantations in Malatya province were surveyed for the presence of Grapevine leafroll-associated viruses (GLRaVs) -1, -2, -3, -4 and -6. Purposive sampling was done in vineyards of Arapgir, Darende and Battalgazi districts, which are the primary grapevine producing areas of Malatya during 2016-2017 growing seasons. A total of 241 samples were collected and tested by DAS-ELISA. Samples giving two fold of absorbance values (405 nm) of negative control were evaluated as positive. According to the results, 131 samples were found to be infected with GLRaV-1, whereas 48 samples with GLRaV-3 and -4 samples with GLRaV-4. None of the samples were reacted as positive against GLRaV-2 and GLRaV-6. Forty five samples were found to be infected with mix infection of GLRaV-1 and -3, and three samples were found to be infected with GLRaV-1 and -4 strain 6.