Potato Wart Disease (PWD) caused by Synchytrium endobioticum is a serious disease affecting potato crop in the infested areas, which has a limited distribution in the world. Although the main approach to control the disease is to restrict its dissemination by legislative measures, growing of resistant varieties is also allowed in the infested areas. To achieve this goal pathotypes of the causal organism, which is more than 40 in Europe, are determined since reactions of the varieties change according to the pathotypes. In this study, pathotypes of fourteen isolates collected from Nevşehir (7), Niğde (5) and Kayseri (2) provinces of Turkey, which are infested by PWD were compared with the Ukrainian isolates by using Saltykova and Yakovleva method employing Ukrainian differential varieties of Poliska rojeva, Svitanok kyivskiy, Volovetska, Kobza, Nesabudka, Kosin-95, Barbara, Bojedar. Two of the isolates (Nevşehir 1 and 5) was also tested before and were not assigned any of the European pathotypes, the rest of them are new isolates. None of the isolates belonged to the Ukrainian pathotypes but 7 pathotypes were differentiated by Ukrainian differentials. These isolates also did not hold the characteristics of Check pathotypes.