Clinostomum complanatum and Clinostomum marginatum (Rudolphi, 1819) (Digenea: Clinostomidae) Are Separate Species Based on Differences in Ribosomal DNA
Copyright policy )Clinostomum complanatum and Clinostomum marginatum (Rudolphi, 1819) (Digenea: Clinostomidae) Are Separate Species Based on Differences in Ribosomal DNA
Infections by metacercariae of Clinostomum (Leidy, 1856) species adversely affect aquacultured fish and are potentially transmissible to humans. Molecular methodologies are efficient tools, which enable diagnosis of all life-history stages of trematodes in their diverse hosts. The small subunit of ribosomal DNA genes of adults of the Old World Clinostomum complanatum (Rudolphi, 1819) and the New World Clinostomum marginatum (Rudolphi, 1819), obtained from a little egret Egretta garzetta (Linnaeus, 1766) and the great blue heron Ardea herodias (Linnaeus, 1758), respectively, were amplified, sequenced, and aligned. The resulting alignment was used to develop a genetic assay to differentiate between these species.
- Hebrew University of Jerusalem Israel
- North Carolina Agricultural and Technical State University United States
- North Carolina State University United States
Polymorphism, Genetic, Base Sequence, Bird Diseases, Molecular Sequence Data, Cyprinidae, Genetic Variation, Trematode Infections, DNA, Helminth, DNA, Ribosomal, Polymerase Chain Reaction, Birds, Fish Diseases, Species Specificity, Animals, Trematoda, Sequence Alignment, DNA Primers
Polymorphism, Genetic, Base Sequence, Bird Diseases, Molecular Sequence Data, Cyprinidae, Genetic Variation, Trematode Infections, DNA, Helminth, DNA, Ribosomal, Polymerase Chain Reaction, Birds, Fish Diseases, Species Specificity, Animals, Trematoda, Sequence Alignment, DNA Primers
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