The 19 genera within the phylum Nematomorpha contain approximately 350 species. The cosmopolitan genus Gordionus Müller, 1926 contains about 58 species, 6 of which occur in the contiguous United States of America. Recently, 2 new Gordionus species were described from high-altitude streams within the southern Rocky Mountains, near Santa Fe, New Mexico. Here we describe another new Gordionus species, from a high-altitude stream in the southern Rocky Mountains, from near Taos, New Mexico. The sites consisted of temporary puddles and a small human-made stream at 3,175-3,250-m altitude in aspen/pine woodland. Gordionus lokeri n. sp. has 1 areole type, which varies in shape and size between and within body regions. Midbody areoles are elongated, polygonal, or triangular, shingled, with the raised side of the areole serrated. The interareolar space is narrow, containing few bristles. The male cloacal opening is surrounded inside and outside by narrow branching bristles that bifurcate or trifurcate deeply; the furcae then subdivide several times terminally. Adhesive warts are lacking. Genetic data, consisting of partial cytochrome c oxidase subunit I (COI) gene sequences, clearly separated G. lokeri n. sp. from other Nearctic species. This is the third Gordionus species described from high-elevation streams in the Rocky Mountains. It appears that this high-altitude habitat represents the preferential niche for numerous species of this genus, and thus future work should focus on describing gordiid diversity in other parts of the Rocky Mountains.