Objective To resolve if TBX22 mutations cause isolated tongue-tie in the Finnish population. Design Mutation analysis of the coding region of the TBX22 gene in 50 Finnish isolated tongue-tie patients and 61 control samples. Results One putative sequence variation was identified from two male patients, but whether this represents a polymorphism or causative mutation remains unknown. Conclusions Mutations in the coding region of the TBX22 gene are not a major cause of aankyloglossia in the Finnish population and do not explain the sex difference or inheritance of tongue-tie.