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Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species

descriptionPublicationkeyboard_double_arrow_right Article 26 Mar 2018Publisher:FapUNIFESP (SciELO)Journal:Neotropical Ichthyology, volume 16 (issn: 1679-6225, eissn: 1982-0224, Copyright policy )Publicly funded
Authors: Caroline Apolinário-Silva; Dhiego G. Ferreira; Analiza F. Cavenagh; Nícollas G. O. Aprígio; Bruno A. Galindo; Jens Carlsson; Silvia H. Sofia;

doi: 10.1590/1982-0224-20170135

Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species

Abstract

ABSTRACT Data on 15 novel microsatellite loci from the Neotropical fish Bryconamericus aff. iheringii are presented here. Analyses of 32 individuals from four different streams revealed 192 different alleles, ranging from four to 32 alleles per locus (mean of 12.8 per locus). Observed and expected heterozygosities ranged from 0.094 to 0.813 and 0.205 to 0.952, respectively. These loci showed high polymorphic information content and will be a resource for genetic studies of B. aff. iheringii. Furthermore, several loci also amplified other small Neotropical Characidae (Piabarchus stramineus and Piabina argentea) and should be useful for these species.

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Keywords

Diversidade genética, Peixe Neotropical, Molecular markers, Biodiversity, Neotropical fish, SSR, Genetic diversity, QL1-991, Lambarizinho, Marcadores moleculares, Zoology, Taxonomy

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