The present study aimed to create an inventory of non-native fish species in the basin of the Sorocaba river, located at Upper Rio Paraná. Fish were collected between August 2010 and January 2012 using nets with meshes, round shaped fishing nets, hand net and electric fishing equipment, as well as contacts with fishermen to seek information on species captured in the basin. Besides, we used published data and information contained in reports, monographs and thesis. Five non-native fish species were collected: Tilapia rendalli, Oreochromis niloticus, Pterygoplichthys anisitsi, Poecilia vivipara and Triportheus nematurus that represented 10.63% of all fish species captured in the basin of the Sorocaba river. The occurrence of eight other species, although they were not collected by the sampling program of this study, had already been reported in previous studies, and had already been captured by amateur and professional fishermen, particularly in the reservoir of Itupararanga and in the urban stretch of the Sorocaba river.