To investigate the effect of Zn fertilization on soil Zn fractions and grain Zn concentration in wheat grown on potentially Zn-deficient soil, a field experiment was carried out. The experimental design was split plot consisted of two varieties of wheat (Zhengmai 9023 and Xinong 889) with five Zn levels (0, 7.5, 15, 30 and 45 kg Zn/ha). Results showed that Zn fertilization had no significant effect on wheat yield and grain Zn concentration, and the recovery of Zn fertilizer was only 0.06% to 0.29%. However, the amount of soil DTPA-Zn was increased by 2.3-9.8-folds as Zn supplementation increases during the whole wheat growth stage as compared to the control (Zn0 treatment). Besides, DTPA-Zn was positively correlated with both Loose organic matter bound Zn (LOM-Zn) and Exchange Zn (Ex-Zn), and their partial correlation coefficients were 0.558 and 0.119, respectively. Moreover, these two fractions also showed positive correlation with grain Zn concentration. The amount of LOM-Zn was firstly increased with i...