Haemotropic Mycoplasma infection among cattle has not been seriously looked into, despite the fact it is associated with poor weight gain and depression. One thousand and forty-five blood samples from apparently healthy cattle from forty-three farms within the entire Peninsular Malaysia were exa­mined over a one-year period. Using polymerase chain reaction to amplify the 16S rRNA gene, the presence of “Candidatus Mycoplasma haemobos” as one of the haemotropic Mycoplasma species that affects cattle in the country was detected. Forty-seven percent of the sampled population was positive for “C. M. haemobos”, with the antigen present in all herds sampled. Breed of cattle, gender, age, production type, physiological status, herd size, management system, farm size, farm age, prophylactic treatment against blood parasites, presence of ticks, frequency of de-ticking, zone, closeness to forest, closeness to waste area, closeness to human settlement and closeness to body of water were all significantly associated (P<0.05) with the detection of “C. M. haemobos”. The findings from this study will be pivotal in government policy on this blood Mycoplasma infection, as the study happens to be the first comprehensive molecular survey of the microorganism in the country.