Juncales is an order of vascular plants with cosmopolitan distribution. Two families of Juncales are present in flora of Ukraine: Juncaceae and Cyperaceae. Through 1985–2021, we conducted comprehensive revision of Juncales in Ukraine. The aim of this research was to compile a checklist of Juncales species in the flora of Ukraine. Our research is based on herbarium materials, literature data and field trips. We collected data in many herbariums of Ukraine and other countries. Our understanding of the taxa was further supplemented by field observations in mountainous (Carpathians and Crimea) and plain territories of Ukraine. Also, we collected information about Juncales species from numerous literature sources. For each taxon, we provided nomenclatural citation and basic synonyms. According to our data, the order Juncales in the flora of Ukraine contains 188 species, belonging to 19 genera. Lists of Juncales species can be found in many thorough publications. For a long time, Checklist of Mosyakin & Fedoronchuk (1999) was the main list in the nomenclature of vascular plants in Ukraine. To date, many nomenclature and taxonomic changes have been accumulated. For example, we accept genera Schoenoplectiella and Oreojuncus here. Researchers have found many new species for the territory of Ukraine. This information can be found in numerous publications, but is fragmented. Therefore, we have compiled an updated summary of the Juncales species. In Ukrainian territory, Juncales species are considered both widespread and rare. We refer to the rarest species Carex alba, C. bicolor, C. bohemica, C. brunnescens, C. buxbaumii, C. depauperata, C. fuliginosa, C. globularis, C. heleonastes, C. lachenalii, C. loliacea, C. obtusata, C. pediformis, C. rupestris, C. strigosa, Cyperus longus, Eleocharis multicaulis, E. oxylepis, Fimbristylis bisumbellata, Juncus acutiflorus, J. soranthus, J. subnodulosus, Isolepis setacea, Luzula spicata, Schoenoplectus pungens, Trichophorum alpinum, T. cespitosum. Also, the following species are rare: Bolboschoenus yagara, Carex chordorrhiza, C. davalliana, C. dioica, C. hostiana, C. pauciflora, C. secalina, C. vaginata, Cladium mariscus, Eleocharis carniolica, Juncus capitatus, J. castaneus, J. littoralis, J. sphaerocarpus, J. thomasii, J. triglumis, Luzula alpinopilosa, L. sudetica, Schoenoplectiella mucronata, Schoenus ferrugineus.