Ficus hispida L. belongs to the Moraceae family and is used by the maaiba trible (indigenous medicine – man of Manipur, India) as an indigenous traditional medicine. Present study deals with the successive extraction of the aerial parts of Ficus hispida and in-vitro screening of anti-oxidant and anti-microbial activity. The phytochemical screening of the methanol extract of Ficus hispida shows the presence of secondary metabolite groups like alkaloid, phenolic compounds, flavonoid, glycosides, protein etc. Phenolic compounds are commonly found in both edible and nonedible plants and are responsible for various medicinal activities of plants, so our study is based on determining antioxidant activity and anti-microbial activity. Beside these, we also measured the total flavonoid and total phenolic content of the respective sample to understand the effect of polyphenolic compound on different pathophysiological state associated with high free radical production. The in-vitro investigation proves the efficiency of this plant in various diseases states.