Phage Display of RNase A and an Improved Method for Purification of Phages Displaying RNases

descriptionPublicationkeyboard_double_arrow_right Article 15 Jan 2000Publisher:Walter de Gruyter GmbHJournal:Biological Chemistry, volume 381 (issn: 1431-6730,

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Authors: K, Korn; H H, Foerster; U, Hahn;

doi: 10.1515/bc.2000.024

pmid: 10746750

Phage Display of RNase A and an Improved Method for Purification of Phages Displaying RNases

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Abstract

Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases.

Related Organizations

Leipzig University
Germany

Keywords

Recombinant Fusion Proteins, Genetic Vectors, Ribonuclease, Pancreatic, Protein Engineering, DNA-Binding Proteins, Animals, Bacteriophages, Capsid Proteins, Cattle, Electrophoresis, Polyacrylamide Gel, Viral Fusion Proteins

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