An update of the “Catalogue of the vascular plants of the Southern Cone” of South America (Argentina, southern Brazil, Chile, Paraguay, and Uruguay) is here summarized, eleven years after the publication of the original treatment. A summary of all Lycophyta, Monilophyta, Gimnospermae, and Angiospermae (Monocotyledoneae and Dictotyledoneae), updated to July 2019, is here presented. This checklist includes nowadays 289 families, 2813 genera, and 18931 species. The number of species is 1238 higher than that registered for the original version, which shows the large number of floristic and taxonomic news published for the area, and the importance of keeping this information updated. Tables sumarizing the floristic information for the Southern Cone and for each country are included, distinguishing by higher groups, families and genera; a comparison with the original version of the catalogue is conducted; endemic families and genera are specified, as well as the introduced ones; finally floristic relationships between the involved countries are evaluated. In addition, two digital appendixes are included, one with the new version of the catalogue, the other with a summary of the flora for each country.