VesMATIC cube80™ is a newly developed automated method for measurement of erythrocyte sedimentation rate (ESR). AIMS: The aim of our study was to compare the ESR values by VesMATIC cube80™ against the modified Westergren method. MATERIALS AND METHODS: This cross-sectional study was conducted at clinical laboratory in Kasturba Hospital, Manipal on 162 patients. The samples taken were as per the recommendations charted out by International Council for Standardization in Hematology (ICSH) for comparing automated and manual Westergren method. STATISTICAL ANALYSIS USED: Bland and Altman statistical analysis was applied for evaluating VesMATIC cube80™ against the conventional Westergren method. RESULTS: The analysis revealed a low degree of agreement between the manual and automated methods especially for higher ESR values (25mm/hr.), i.e., mean difference -0.44 (95% limits of agreement, -41.23 to 40.37). This discrepancy which is of clinical significance was less evident for ESR values in the normal range <25 mm/hour (-1.73 mean of difference; -18.0 to 10 limits of agreement). CONCLUSIONS: The fully automated system VesMATIC cube80™ for ESR measurement tends to underestimate the manual ESR readings. Further studies and validation experiments would be required.