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Sorghum (<i>Sorghum bicolor</i> L.)

Authors: Zuo-yu Zhao;

Sorghum (<i>Sorghum bicolor</i> L.)

Abstract

This chapter describes a stepwise protocol for Agrobacterium-mediated sorghum genetic transformation. Immature embryos from sorghum plants were used as the target explants. The Agrobacterium strain LBA4404, carrying a "super-binary" vector, was used in this protocol. Agrobacterium co-transformation vectors, one T-DNA containing the selectable marker gene and another T-DNA containing the trait gene(s), were also introduced in sorghum transformation for eliminating the selectable marker gene in the resulting transgenic plants. This chapter provides recommendations for analysis of the transgenic plants to confirm T-DNA integration into the sorghum genome and segregation of the selectable marker gene from the trait gene(s).

Related Organizations
Keywords

DNA, Bacterial, Genetic Markers, Gene Transfer Techniques, Sequence Analysis, DNA, Plants, Genetically Modified, Mutagenesis, Insertional, Quantitative Trait, Heritable, Transformation, Genetic, Agrobacterium tumefaciens, Genome, Plant, Sorghum

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    citations
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    57
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 10%
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Found an issue? Give us feedback
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
57
Top 10%
Top 10%
Top 10%
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