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Genotyping SNPs With the LightCycler

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 29 Nov 2004Publisher:Humana Press

Authors: Maria Victoria Lareu; Clara Ruiz-Ponte;

doi: 10.1385/1-59259-867-6:127

pmid: 15570104

Genotyping SNPs With the LightCycler

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Abstract

Here, a single nucleotide polymorphism typing methodology is described based on polymerase chain reaction monitoring, in real time, of fluorescently labeled amplified products using the LightCycler. The main advantages of the system are the time required for the analysis (about 20 min), combined with the robustness, accuracy, and the sensitivity of the method.

Related Organizations

University of Santiago de Compostela
Spain

Keywords

Chromosomes, Human, Y, Base Sequence, Genotype, Humans, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, DNA Primers, Fluorescent Dyes

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	3
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average