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Optics Express
Article . 2025 . Peer-reviewed
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Optics Express
Article . 2025 . Peer-reviewed
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On-the-fly adaptive SNR protocol to accelerate Brillouin microscopy

Authors: Brechet, Léo; Gilet, Valentin; Bouhlel, Nizar; Loumaigne, Matthieu; Ruckebusch, Cyril; Dobigeon, Nicolas; Zhang, Li; +5 Authors

On-the-fly adaptive SNR protocol to accelerate Brillouin microscopy

Abstract

This article presents an innovative method for accelerating Brillouin microscopy imaging. The proposed technique, called One-pass, dynamically adjusts the signal-to-noise ratio (SNR) during data acquisition. It identifies essential spectra in real-time and adapts the laser exposure time accordingly. This approach considerably reduces acquisition time (by a factor around 2.2 and 3.5) while maintaining acceptable image quality, as demonstrated on simulations and real biological samples (HeLa and SK-N-SH cells). The method improves the stability and efficiency of Brillouin imaging compared with conventional techniques, i.e., raster scan with a single long exposure time or the recently introduced two-pass approach.

Country
France
Keywords

[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics], [INFO.INFO-TI] Computer Science [cs]/Image Processing [eess.IV]

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green
gold
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