We report on the first injection-seeded nanosecond Ti:Sapphire laser that demonstrates a stable single-longitudinal-mode operation with no feedback loop for active cavity stabilization. The short cavity generates 6-mJ transform-limited pulses at a wavelength of 807 nm and with a slope efficiency of 43%. An intracavity dispersive prism makes a novel cavity design for injection-seeded lasers and provides pre-selection of the emission wavelength. In support of these experiments, we perform numerical simulations that include extra cavity losses. The simulation results are in good agreement with the outcome of the experiment and reveal the formation scenario of the laser mode.