We analyzed the fungi efficiency in the biological control of witches’ broom disease in different experimental conditions (laboratory, greenhouse, and field) through a systematic review of the literature. Species/isolates were divided into three groups and the mean efficiency of these microorganisms was assessed. The groups were represented by the genera Trichoderma (51.5% efficiency) and Clonostachys (38%), and the endophytic fungi (22%). Nevertheless, experimental conditions may affect the biological control efficiency. Trichoderma and Clonostachys were the most efficient genera in reducing the incidence of witches´ broom disease and also the most used in biocontrol this phytopathogenicity.