Molecular Characterization of Sucrose:Sucrose 1-Fructosyltransferase and Sucrose:Fructan 6-Fructosyltransferase Associated with Fructan Accumulation in Winter Wheat during Cold Hardening
Copyright policy )Molecular Characterization of Sucrose:Sucrose 1-Fructosyltransferase and Sucrose:Fructan 6-Fructosyltransferase Associated with Fructan Accumulation in Winter Wheat during Cold Hardening
We isolated two cDNAs of winter wheat (Triticum aestivum L.), designated wft1 and wft2, which encoded sucrose:fructan 6-fructosyltransferase (6-SFT) and sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99), respectively, which are involved in the synthesis of fructan in wheat. wft1 and wft2 were cloned by screening of a cDNA library with probed-cDNA fragments corresponding to plant fructosyltransferase and invertase. The identity of the clones was verified by functional characterization of recombinant proteins expressed in methylotrophic yeast, Pichiapastoris. Northern blotting showed that the level of wft2 transcripts increased from autumn to early winter in the crown tissues of all field-grown wheat cultivars examined. Higher levels of wft1 and wft2 transcripts were found in leaf tissues of snow mold-resistant cultivars, which accumulated more fructan than other cultivars. Our results showed that Wft1 and Wft2 were important in fructan accumulation during cold hardening of winter wheat.
Sucrose, Binding Sites, DNA, Complementary, Glycoside Hydrolases, beta-Fructofuranosidase, Molecular Sequence Data, Carbohydrates, Blotting, Northern, Pichia, Recombinant Proteins, Fructans, Cold Temperature, Plant Leaves, Hexosyltransferases, Consensus Sequence, Seeds, Amino Acid Sequence, Seasons, Cloning, Molecular, Triticum
Sucrose, Binding Sites, DNA, Complementary, Glycoside Hydrolases, beta-Fructofuranosidase, Molecular Sequence Data, Carbohydrates, Blotting, Northern, Pichia, Recombinant Proteins, Fructans, Cold Temperature, Plant Leaves, Hexosyltransferases, Consensus Sequence, Seeds, Amino Acid Sequence, Seasons, Cloning, Molecular, Triticum
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