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Bioscience Biotechnology and Biochemistry
Article . 2013 . Peer-reviewed
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Improvement of the Lipophilic-Oxygen Radical Absorbance Capacity (L-ORAC) Method and Single-Laboratory Validation

Authors: WATANABE, Jun; OKI, Tomoyuki; TAKEBAYASHI, Jun; YAMASAKI, Koji; TAKANO-ISHIKAWA, Yuko; HINO, Akihiro; YASUI, Akemi;

Improvement of the Lipophilic-Oxygen Radical Absorbance Capacity (L-ORAC) Method and Single-Laboratory Validation

Abstract

We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used. The antioxidant capacities of food extracts can be evaluated by this method with nearly the same precision as antioxidant solutions.

Keywords

Spectrometry, Fluorescence, Free Radicals, lipophilic oxygen radical absorbance capacity, method validation, Reproducibility of Results, antioxidant capacity, Free Radical Scavengers, Laboratories, Hydrophobic and Hydrophilic Interactions

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    popularity
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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
20
Top 10%
Top 10%
Top 10%