Rhodes, Symi and Chalki are three islands with different characteristics in the southeastern part of the Aegean Sea. In this work, we present their complete land snail fauna based on recent survey, samples from the collections of the Natural History Museum of Crete and a thorough bibliographic review. We report 67 land snail species from Rhodes, 34 species from Symi, and 32 from Chalki, with 4, 20 and 9 new records for each island, respectively. Also, one species has been added to the malacofauna of Greece, while many names, which were mentioned mainly in the 19th century, have been clarified, synonymised or abolished from the lists of the islands. Our results once again demonstrate the importance of the reproductive system as a tool for reliable assessment of the taxonomy of Aegean land snails, and that sampling during the rainy season should be the preferred methodological approach for collecting land snails in the Greek islands.