Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation
Copyright policy )Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation
The eukaryotic translation initiation factor 1 binds to the ribosome during translation initiation. It is instrumental for initiator-tRNA and mRNA binding, and has a function in selection of the authentic start codon. Here, we show that the archaeal homolog aIF1 has analogous functions. The aIF1 protein of the archaeon Sulfolobus solfataricus is bound to the small ribosomal subunit during translation initiation and accelerates binding of initiator-tRNA and mRNA to the ribosome. Accordingly, aIF1 stimulated translation of an mRNA in a S. solfataricus in vitro translation system. Moreover, this study suggested that the C terminus of the factor is of relevance for its function.
- Vienna Biocenter Austria
- University of Vienna Austria
- University of Bari Aldo Moro Italy
- Sapienza University of Rome Italy
- Roma Tre University Italy
106022 Mikrobiologie, Base Sequence, aif1; archaea; sulfolobus solfataricus; translation initiation, Molecular Sequence Data, Eukaryotic Initiation Factor-1, RNA, Archaeal, Sulfolobus, Genes, Bacterial, Protein Biosynthesis, 106022 Microbiology, RNA Interference, Amino Acid Sequence, Guanosine Triphosphate, Cloning, Molecular, Ribosomes, DNA Primers, Protein Binding
106022 Mikrobiologie, Base Sequence, aif1; archaea; sulfolobus solfataricus; translation initiation, Molecular Sequence Data, Eukaryotic Initiation Factor-1, RNA, Archaeal, Sulfolobus, Genes, Bacterial, Protein Biosynthesis, 106022 Microbiology, RNA Interference, Amino Acid Sequence, Guanosine Triphosphate, Cloning, Molecular, Ribosomes, DNA Primers, Protein Binding
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