Discovery of Novel Adenylyl Cyclase Inhibitor by Cell-Based Screening
Copyright policy )Discovery of Novel Adenylyl Cyclase Inhibitor by Cell-Based Screening
We screened 2400 compounds to find novel inhibitors of the adenylyl cyclase (AC)-protein kinase A (PKA)-cAMP response-element-binding protein (CREB) signaling pathway (AC/PKA/CREB pathway). Using a multistep cell-based screening system employing split luciferase technique, we narrowed down the candidates effectively from 2400 chemical compounds and identified a novel AC inhibitor (compound 1). Since dysregulation of the AC/PKA/CREB pathway is known to cause diseases not only in the nervous system but also in other organs, compound 1 is expected to be developed as a medicine for these diseases.
- University of Toyama Japan
HEK293 Cells, Dose-Response Relationship, Drug, Adenylyl Cyclase Inhibitors, Drug Discovery, Drug Evaluation, Preclinical, Humans, Enzyme Inhibitors, Adenylyl Cyclases
HEK293 Cells, Dose-Response Relationship, Drug, Adenylyl Cyclase Inhibitors, Drug Discovery, Drug Evaluation, Preclinical, Humans, Enzyme Inhibitors, Adenylyl Cyclases
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