
doi: 10.1186/bf03547344
The metabolism of intravenously injected acetate-1-C14 in bovine fasting ketosis was compared with the acetate metabolism in normal cows and in cows with spontaneous ketosis. The mean standardized specific activity in respired CO2 over the first 3 hours was 18.9 compared with 16.2 for ketotic and 11.0 for normal cows. The mean standardized specific activity in blood glucose and urine ketone bodies over the first 4 hours was 1.88/7.9 compared with 1.29/7.4 in ketotic and 1.09/2.5 in normal cows. Furthermore the mean standardized specific activity of milk citrate, lactose, casein and albumin over the first 48 hours was greater in fasting ketosis than in the normal cows giving a picture similar to that found during spontaneous ketosis. The total C14 recovered in the milk fat within 48 hours was about 4 per cent compared with 14 per cent in the normal cow and 2 per cent during spontaneous ketosis. These results suggest that the metabolic lesions in spontaneous and fasting ketosis are very much alike. The results support the theory of impaired lipogenesis and contradict the assumption of decreased tricarboxylic acid cycle activity during bovine ketosis. Omsaetningen af intravenost injiceret 1-C14 maerket acetat er blevet undersogt hos kvaeg med sultketose og resultaterne sammenlignet med lignende forsog udfort pa normale koer og pa koer med spontan ketose. Den gennemsnitlige specifikke aktivitet i udandingsluftens CO2 i de forste tre timer efter injektionen blev fundet til 18.9 sammenlignet med 16.2 for koer med spontan ketose, og 11.0 for normale koer. Forholdet mellem den gennemsnitlige specifikke aktivitet i blodets glukose og urinens ketonstoffer i de forste 4 timer efter injektionen var 1.88/7.9 sammenlignet med 1.29/7.4 for koer med spontan ketose og 1.09/2.5 hos normale koer. Endvidere var den gennemsnitlige specifikke aktivitet i maelkens indhold af citrat, lactose, casein og albumin i de forste 48 timer efter injektionen storre end hos normale koer, men svarende ganske til de vaerdier, som er fundet ved spontan ketose. — Den maengde C14, som kunne genifindes i maelkens fedt i de forste 48 timer, var omkring 4 % sammenlignet med ca. 14 % hos normale koer og ca. 2 % hos koer med spontan ketose. Disse resultater tyder pa, at de stofskifteforstyrrelser, som findes under spontan ketose og ved sultketose er af samme karakter. Resultaterne understotter endvidere teorien om „impaired lipogenesis”, og gar imod den antagelse, at trikarboxylsyrecy klubaktiviteten skulle vaere nedsat under ketose.
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