Zoysiagrass is an important ornamental and turfgrass cultivated in different countries. Recently, damage to this plant, characterized by a type of witch’s broom and stunting were observed in southern Brazil, suggesting possible attack by mites. A detailed examination of these plants showed the presence of a new mite species, Abacarus zoysiae Flechtmann & Tassi sp. nov., as responsible for the damage. The objective of this publication is to describe that new species, based on adult females and males.