Objective. Gastrointestinal disfunction and acute lung injury (ALI) were common in acute pancreatitis (AP). The effect of electro‐acupuncture (EA) on gastrointestinal motility and ALI in rats with AP was investigated to verify the theory of “lung and large intestine are interior exteriorly related” in traditional Chinese medicine. Methods. Male Sprague‐Dawley rats were randomly divided into the normal group, model group, and EA group. AP model was established by three injections of 20% L‐arginine at 1 h intervals. EA were applied to bilateral ST‐25 and ST‐36 for 30 minutes twice a day after modeling for 3 days. Arterial blood, pancreas, lung, and intestinal tissues were collected for detecting the inflammatory factors and histopathology. Intestinal propulsion rate (IPR) was also measured at 72 h. Results. EA treatment improved IPR and increased CCK‐8 level compared with model group (P < 0.05). It lowered the serum levels of TNF‐α and IL‐6 and increased the level of IL‐4 with no effect on IL‐10. EA treatment reduced serum vasoactive intestinal peptide (VIP) and myeloperoxidase (MPO) level in the lung and the pathologic scores of pancreas, lung and intestine were decreased (P < 0.05). Conclusion. EA treatment could promote gastrointestinal motility through inhibiting VIP, and promoting CCK expression and regulate pro‐ and anti‐inflammatory mediators to ameliorate ALI in AP.