Pantoeasp. Isolated from Tropical Fresh Water ExhibitingN-Acyl Homoserine Lactone Production
Copyright policy )Pantoeasp. Isolated from Tropical Fresh Water ExhibitingN-Acyl Homoserine Lactone Production
N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production usingChromobacterium violaceumCV026 andEscherichia coli[pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity toPantoea stewartiiS9-116, which is a plant pathogen. The isolatedPantoeasp. was confirmed to produceN-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry.
- Universiti Malaysia Terengganu Malaysia
- University of Malaya Malaysia
Technology, Tropical Climate, Base Sequence, Pantoea, Sequence Analysis, RNA, T, Science, Chromobacterium, Q, Molecular Sequence Data, R, Malaysia, Quorum Sensing, Sequence Homology, Fresh Water, 4-Butyrolactone, Tandem Mass Spectrometry, RNA, Ribosomal, 16S, Escherichia coli, Homoserine, Medicine, Research Article
Technology, Tropical Climate, Base Sequence, Pantoea, Sequence Analysis, RNA, T, Science, Chromobacterium, Q, Molecular Sequence Data, R, Malaysia, Quorum Sensing, Sequence Homology, Fresh Water, 4-Butyrolactone, Tandem Mass Spectrometry, RNA, Ribosomal, 16S, Escherichia coli, Homoserine, Medicine, Research Article
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