We obtain the general solution of the generalized quartic functional equationf(x+my)+f(x-my)=2(7m-9)(m-1)f(x)+2m2(m2-1)f(y)-(m-1)2f(2x)+m2{f(x+y)+f(x-y)}for a fixed positive integerm. We prove the Hyers-Ulam stability for this quartic functional equation by the directed method and the fixed point method on real Banach spaces. We also investigate the Hyers-Ulam stability for the mentioned quartic functional equation in non-Archimedean spaces.