Carotid-to-radial pulse wave velocity (PWVcr) has been proposed to evaluate endothelial function. However, the measurement ofPWVcris not without limitations. A new simple approach could have wide application.Stiffness index(SI) is obtained by analysis of the peripheral pulse wave and gives reproducible information about stiffness of large arteries. This study assessed the effects of hyperemia on SI and compared it withPWVcrin 14 healthy subjects. Both were measured at rest and during 8 minutes after ischemia. SI temporal course was determined. At 1 minute, SI andPWVcrdecreased (5.58±0.24to5.34±0.23 m/s,P<0.05;7.8±1.0to7.2±0.9 m/s;P<0.05, resp.). SI was positively related toPWVcrin baseline (r=0.62,P<0.05), at 1 minute (r=0.79,P<0.05), and during the whole experimental session (r=0.52,P<0.05).Conclusion. Hyperemia significantly decreases SI in healthy subjects. SI was related toPWVcrand could be used to facilitate the evaluation of hyperemia-related changes in arterial stiffness.