Better knowledge of aerobiological properties of pollen clouds is needed for pines and other woody perennials because these properties shape the predictive accuracy of spatially explicit pollen dispersal models. Four properties were experimentally measured in this study using processed Pinus taeda L. (loblolly pine) pollen as well as pollen concentration and viability data collected from an 18-year-old P. taeda plantation. Results showed that P. taeda pollen had a settling or terminal velocity value of 2.1 cm·s–1; that daytime pollen count from a plantation was sparse, reaching a maximum of 1480 grains·m–3 at peak pollen shed; and that pollen concentration showed no vertical gradient above or below the plantation’s canopy. Pollen sampled above and within the canopy had comparable germination rates. Surprisingly, a low concentration of viable Pinus spp. pollen was present during nighttime. Study results added further to the idea that P. taeda pollen has a higher nuisance value than either Zea mays L. (maize) or Agrostis stolonifera L. (creeping bentgrass) pollen because of its low terminal velocity value, persistent viability, and perennial production.