Capturing Greater 16S rRNA Gene Sequence Diversity within the Domain Bacteria
Copyright policy )Capturing Greater 16S rRNA Gene Sequence Diversity within the Domain Bacteria
ABSTRACT A large proportion of “universal” 16S PCR primers lack sequence homology to many of the “candidate” divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of taxonomic diversity with around a 3-fold increase in recovery of sequences from candidate divisions.
- UNSW Sydney Australia
DNA, Bacterial, Bacteria, Genetic Variation, Genes, rRNA, Sequence Analysis, DNA, DNA, Ribosomal, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Metagenomics, Soil Microbiology, DNA Primers
DNA, Bacterial, Bacteria, Genetic Variation, Genes, rRNA, Sequence Analysis, DNA, DNA, Ribosomal, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Metagenomics, Soil Microbiology, DNA Primers
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